quantikine elisa (R&D Systems)

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R&D Systems quantikine elisa

IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or <t>ELISA.</t> Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001

Quantikine Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cxcl1/Human+CXCL1%2FGRO+alpha+Quantikine+ELISA+Kit/pmc13107899-54-11-15
Average 94 stars, based on 51 article reviews

quantikine elisa - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling"

Article Title: IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling

Journal: Stem Cell Research & Therapy

doi: 10.1186/s13287-026-05029-x

Figure Legend Snippet: IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or ELISA. Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001

Techniques Used: Expressing, Control, Enzyme-linked Immunosorbent Assay, Derivative Assay

other:

Article Title: A chemokine regulatory loop induces cholesterol synthesis in lung-colonizing triple-negative breast cancer cells to fuel metastatic growth
Article Snippet: Membranes were then incubated with IRDye 680CWor IRDye 800CW secondary antibodies (LI-COR Biosciences) for 1 h at room temperature.

Recombinant:

Article Title: Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7
Article Snippet: .. For recombinant protein stimulation, CASMCs were treated with human CCL17 (ab283903; Abcam, Cambridge, UK) at 100 nM and/or 500 nM (as indicated in the respective figure legends) for 24 h, similarly for all: human IL-6 (206-IL-010; R&D Systems, Minneapolis, MN, USA), human CXCL1 (275-GR; R&D Systems), and human CCL2 (279-MC; R&D Systems). ..

Article Title: Structure–activity studies of Streptococcus pyogenes enzyme SpyCEP reveal high affinity for CXCL8 in the SpyCEP C-terminal
Article Snippet: .. To visualise chemokine cleavage, 50 pmol of recombinant human CXCL1 or CXCL8 (R&D Systems) was incubated with C-terminal SpyCEP in a final volume of 20 μl reaction buffer (40 mM Tris-HCL pH 7.5, 0.1% BSA, 75 mM NaCl), at a molar ratio of 1:5, 1:50, 1:500, 1:5000 in favour of chemokine for 2 h at 37 °C. ..

Article Title: Structure-activity studies of Streptococcus pyogenes enzyme SpyCEP reveal high affinity for CXCL8 in the SpyCEP C-terminal.
Article Snippet: .. To visualise chemokine cleavage, 50 pmol of recombinant human CXCL1 or CXCL8 (R&D Systems) was incubated with C-terminal SpyCEP in a final volume of 20 μl reaction buffer (40 mM Tris-HCL pH 7.5, 0.1% BSA, 75 mM NaCl), at a molar ratio of 1:5, 1:50, 1:500, 1:5000 in favour of chemokine for 2 h at 37 °C. ..

Incubation:

Enzyme-linked Immunosorbent Assay:

Article Title: Paracrine Factors of Stressed Peripheral Blood Mononuclear Cells Activate Proangiogenic and Anti-Proteolytic Processes in Whole Blood Cells and Protect the Endothelial Barrier
Article Snippet: .. Protein levels of Human CXCL1, human CXCL5, human SERPINB2, human VEGF-A, and human urokinase (R&D Systems, Biotechne, Minneapolis, MN, USA) were quantified by ELISA as recommended by the manufacturer. .. Absorbance was measured at 450 nm by a Spark multimode microplate reader (Tecan, Männedorf, Switzerland), and analyte quantifications were determined using external standard curves.

Article Title: Apolipoprotein E promotes immune suppression in pancreatic cancer through NF-κB-mediated production of CXCL1
Article Snippet: ApoE in mouse macrophage media was measured with Mouse Apolipoprotein E SimpleStep ELISA Kit (Abcam, ab215086). .. Human CXCL1 was measured with Human CXCL1/GRO alpha Quantikine ELISA Kit (R&D systems, DGR00B). ..

Sandwich ELISA:

Article Title: The NR4A Orphan Receptor Modulator C-DIM12 Selectively Alters Inflammatory Mediators in Myeloid Cells
Article Snippet: .. This is a sandwich ELISA technique with a pre-coated antibody on a given microplate, which was specific to human CXCL1 (R&D system, Cat DGR00B). ..

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